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. Author manuscript; available in PMC: 2017 Sep 25.
Published in final edited form as: Int J Pharm. 2016 Aug 2;511(2):1012–1021. doi: 10.1016/j.ijpharm.2016.07.071

Figure 3. Dose dependent cytotoxicity of CNGs towards HUVECs after 24 hours of treatment and comparison with free curcumin.

Figure 3

(A) Cell viability calculated after carrying out Calcein AM red-orange live cell tracer assay (B) Mitochondrial oxygen consumption rate profile of a typical mitochondrial stress assay conducted using Seahorse XF96 instrument. (C) Basal respiration rates of curcumin and CNGs represented at pmol/min after the calculations as depicted in Figure 3A. N=5 replicates were used for each group. (D) OCR (profile) of the controls and free curcumin or CNG at different curcumin concentrations in response to mitochondrial stress assay reagents added in a series at selected time points. Error bars: (A) – std. dev.; (C), (D) – std. err.