Fig. 3.

The multilocus RcGTA “genome” and putative roles of its genes in particle production and gene transfer activity. Individual ORFs are drawn to scale, whereas distances between loci are shown in kilobases. The ORFs are shaded in gray if they were not found associated with RcGTA particles in a previous proteomic study (Chen et al. 2008). ORFs that were experimentally tested for their requirement in RcGTA activity are marked by one of the two hatch patterns (references for specific studies are in table 1). The indicated putative functions for genes outside of the structural cluster are discussed in detail in the text.