Fig. 5.

Roles of rcc01865 and rcc01866 in RcGTA production. (A) The relative amounts, as visualized by a representative western blot, of RcGTA capsid protein in cells and culture supernatants of the indicated strains. No capsid protein was detectable within SB1865 cells. In contrast, SB1866 cells contained a considerable amount of the capsid protein, but appeared incapable of releasing RcGTA particles from the cells. (B) RcGTA gene transfer activity released from cells by manual lysis. The gene transfer activity was determined as an average relative to the gene transfer activity from a lysed culture of SB1003 (the dotted line), in three replicate bioassays. No gene transfer was detected in the SB1865 cells. The RcGTA capsid protein accumulated within SB1866 cells does not correspond to functional RcGTA particles because very little gene transfer activity was detected when these cells were manually lysed. The error bars represent the standard deviation. An asterisk (*) indicates that the RcGTA gene transfer activities differed significantly from the control (ANOVA and Tukey HSD; P < 0.001).