Fig. 4.

Comparative analysis of endogenous ligand mediated DNA binding potential and transcriptional activity of the Neandertal and human AHR. (A) Structures of AHR ligands tested. (B) In vitro translated AHR from the appropriate species and ARNT were utilized in electrophoretic mobility shift assays to assess AHR ligand-mediated AHR/ARNT/DNA complex formation. (C) Dose-dependent induction of Cyp1a1 and Cyp1b1 expression mediated by the Neandertal or human AHR in transfected BP8 cells. (D) Summary of the EC₅₀ values for the dose-response experiments in panel C.