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. 2016 Sep 16;11(9):e0162981. doi: 10.1371/journal.pone.0162981

Fig 4. ChtD, ChtE and Srt recombinant proteins were expressed and purified.

Fig 4

(A) The cell pellets of E. coli overexpressing the His-ChtD, His-ChtE and His-Srt recombinant proteins. The red colored cell pellets containing His-ChtD and His-ChtE indicate the presence of heme. (B) Purified His-ChtD (77 kDa), His-ChtE (25 kDa) and His-Srt (20 kDa) proteins were analyzed on a Coomassie-stained 10% SDS-PAGE gel. The band sizes are indicated on the right and the protein marker (M) sizes are shown on the left of the gel.