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. 2016 Sep 16;11(9):e0162981. doi: 10.1371/journal.pone.0162981

Fig 5. ChtD and ChtE recombinant proteins bind heme.

Fig 5

(A) Each purified recombinant protein and a hemin only sample was analyzed by absorbance spectroscopy between wavelengths of 250 to 650 nm. The presence of heme bound to the proteins was determined by a Soret absorbance at ~400 nm. (B) Detection of heme by LC-MS analysis of each purified recombinant protein, a hemin only sample and Tris buffer as a control. A single charged heme species, indicated by the arrow (→), is predicted to have a mass of 616 kDa.