Table 1. Bacterial strains and plasmids used in the study of the cht locus.

Strains or plasmid	Characteristics	Reference
Strains
JIR325	RifR NalR derivative of C. perfringens strain 13	[29]
JIR12755	JIR325 ΔchtDchtE Ω cat(P)	This study
JIR12773	JIR12755 Δcat(P) Ω ermBchtD+chtE+	This study
JIR12614	JIR325 srt::erm(B)	This study
JIR12861	JIR12614 (pJIR4151)	This study
Plasmids
pET28a(+)	E. coli expression vector, N-terminal 6x His-tag, KanR	Novagen
pJIR750	E. coli-C. perfringens shuttle vector, CmR	[30]
pJIR751	E. coli-C. perfringens shuttle vector, EmR	[30]
pJIR2715	Suicide vector base for C. perfringens, EmR	[31]
pJIR2783	Suicide vector base for C. perfringens, CmR, ErmR	[32]
pJIR3562	Targetron vector base for C. perfringens, CmR, ErmR	[33]
pJIR3566	Targetron vector base for C. perfringens, lacZ’, CmR, ErmR	[33]
pJIR3924	pCR-BluntII-TOPO carrying 2.1 kb chtE downstream sequence, KanR	This study
pJIR3926	pCR-BluntII-TOPO carrying 2 kb chtD upstream sequence, KanR	This study
pJIR3928	pJIR2783 with 2 kb BamHI-PstI chtD upstream fragment, CmR, EmR	This study
pJIR3929	pJIR3928 with 2.1 kb SacI-XhoI chtE downstream fragment, CmR, EmR	This study
pJIR4029	pJIR751 with 5.3 kb Asp718-SphI chtD to 650 bp of chtA fragment, EmR	This study
pJIR4032	pJIR3926 with 1.4 kb PstI-NotI erm(Q) cassette of pJIR2715, KanR	This study
pJIR4038	pJIR3566 carrying 353 bp BamHI/HindIII fragment targeted to srt, lacZ’, CmR, ErmR	This study
pJIR4053	pCR-BluntII-TOPO carrying 5.4 kb SacI-PvuI chtD to 650 bp of chtA fragment from pJIR4029, KanR	This study
pJIR4055	pJIR4053 with 3.5 kb SacI isdA upstream sequence and erm(Q) cassette of pJIR4032, KanR	This study
pJIR4151	pJIR750 with 5.2 kb Asp718-SphI chtD to chtA gene fragment, CmR	This study
pJIR4080	Expression vector, pET28a(+) with 2 kb NheI-XhoI chtD gene fragment, KanR	This study
pJIR4075	Expression vector, pET28a(+) with 486 bp NdeI-BamHI chtE gene fragment, KanR	This study
pJIR4077	Expression vector, pET28a(+) with 477 bp NdeI-BamHI srt gene fragment, KanR	This study