Table 2. Distribution of SNPs in the chtDE and srt strains.
Nucleotide change (comparison to wild-type sequence | Genome position | Amino acid change | Putative protein function | ||
---|---|---|---|---|---|
ΔchtDE | ΔchtDE (KI) | srtΩermB | |||
CTG deletion | CTG deletion | CTG deletion | 356016–356018 | A163- | Ferrodoxin hydrogenase (CPE0276) |
C → A | C → A | 652650 | P10Q | ABC transporter (CPE0526) | |
C → A | C → A | 714375 | L371I | Two component sensor histidine kinase YesM (CPE0574) | |
C → A | C → A | 850962 | A102D | Amino phosphoribosyltransferase (CPE0683) | |
C → A | 1051900 | F205L | NADH-dependent butanol dehydrogenase or iron-containing alcohol dehydrogenase (CPE0858) | ||
G → T | 1169378 | E602D | Hypothetical protein (CPE0969) | ||
G → T | 1339585 | E134D | Triphosphoribosyl-dephospho-CoA synthase, CitG (CPE1145) | ||
G → T | 1381825 | M1I | Phosphofructokinase (CPE1185) | ||
C → A | 1931454 | A44D | FeoA (CPE1659) | ||
- → C | - → C | - → C | Insertion after 2292378 | Frameshift | Hypothetical protein (CPE2000) |
C → A | 2323940 | A559D | DnaK molecular chaperone (CPE2033) | ||
G → T | G → T | 2644353 | A54S | Electron transfer flavoprotein beta-subunit (CPE2299) | |
G → T | 2716228 | E405* | Phosphoenolpyruvate-protein phosphotransferase (CPE2357) | ||
G → A | G → A | G → A | 2847860 | D443N | Two component sensor histidine kinase, BaeS (CPE2487) |
* stop codon, KI- complemented in cis, ΩermB- insertional inactivation using the erm(B) gene