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. 2016 Sep 16;14(9):e1002543. doi: 10.1371/journal.pbio.1002543

Fig 2. Sam68 deficiency attenuates DNA damage-initiated repair signaling in cell culture.

Fig 2

(A, E) WT and Sam68 KO MEFs expressing red fluorescent protein (RFP) or RFP-Sam68 were γ-irradiated (γ-irradiation, IR) at 10 Gy, and whole cell lysates were derived at indicated time points and immunoblotted (immunoblot, IB) for indicated proteins, with β-actin as a loading control. (B, D, F) Primary thymocytes isolated from Sam68+/- and Sam68-/- mice were γ-irradiated at 4 Gy, and whole cell lysates were derived at indicated time points and immunoblotted for indicated proteins, with β-actin as a loading control. (C) Immunofluorescence micrographs of PAR and γH2AX in thymocytes collected at 15 min post mock- or γ-irradiation (IR, 4 Gy), with nuclei counterstained by 4′, 6-diamidino-2-phenylindole (DAPI). Scale bar, 10 μm.