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. 2016 Aug 18;1(13):e87023. doi: 10.1172/jci.insight.87023

Figure 6. Injury-induced cytokine expression and kidney ingress of macrophages and neutrophils is reduced in vivo in ADAM17ex/ex and ADAM17 PTC-KO mice.

Figure 6

(A–C) The mRNA expression of TGFβ, MCP1, and PDGFRβ was examined by qPCR in whole kidney lysates of WT/WT or ex/ex mice at different time points after ischemia (n = 4). (D–G) Macrophage and neutrophil infiltration in the kidney of ADAM17ex/ex mice (D and E; n = 5–6) or PTC-KO (Cre fl/fl) mice (F and G; n = 4–5) and their WT/WT littermates was examined by immunostaining at different time points after ischemia or 7 days after UUO (n = 5). *P < 0.05; **P < 0.01;***P < 0.001 as determined by an unpaired 2-tailed Student’s t test. ADAM17, a disintegrin and metalloprotease 17; IRI, ischemia reperfusion injury; MCP1, monocyte chemoattractant protein 1; PDGFRβ, platelet derived growth factor receptor beta; PTC-KO, proximal tubule cell KO; UUO, unilateral ureteral obstruction.