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. 2016 Aug 30;5:e16420. doi: 10.7554/eLife.16420

Figure 3. Neural representation of different sensory modalities after cue-taste association.

(A) Percentage of neurons (n = 118) modulated (excited [gray] or inhibited [white]) by each tastants and anticipatory cues. Asterisks indicate post-hoc test corrected for multiple comparisons (Marascuillo’s test, p<0.05). (B) Pie chart showing the proportion of taste selective neuron (n = 68) that are modulated exclusively by taste (Taste only) or by taste and one (Taste & 1), two (Taste & 2), three (Taste & 3) or four (Taste & 4) anticipatory cues. (C) Pie chart showing the proportion of cue responsive neuron (n = 69), which are only modulated by one (1 Cue), two (2 Cues), three (3 Cues) or all anticipatory cues (4 Cues). (D) Raster plot and PSTH of a representative GC neuron showing a significant excitatory response to sucrose. (E) Raster plot and PSTH of a GC neuron (same as the one showed in panel D) featuring significant excitatory responses to Air puff and Odor and no responses to Tone and Light. (F) Raster plot and PSTH of a GC neuron showing inhibitory responses to multiple tastants. (G) Raster plot and PSTH of a GC neuron (same as the one showed in panel F) displaying significant inhibitory responses to multiple cues (air puff, odor, tone and light). For D and F: vertical lines at time 0 indicate the onset of the taste delivery. S for sucrose, N for NaCl, C for citric acid and Q for quinine. For E and G: gray rectangular areas indicate the period of cue presentation. Vertical dotted lines at time 2.5 indicate the onset of sucrose delivery. Red dotted line represents the time-course of orofacial movement activity.

DOI: http://dx.doi.org/10.7554/eLife.16420.009

Figure 3—source data 1. Percentage of GC neurons responding to cross-modal and gustatory stimuli in trained rats.
DOI: http://dx.doi.org/10.7554/eLife.16420.010
elife-16420-fig3-data1.xlsx (16.4KB, xlsx)
DOI: 10.7554/eLife.16420.010

Figure 3.

Figure 3—figure supplement 1. Electrode placement and experimental design for the group of trained rats.

Figure 3—figure supplement 1.

(A) Schematic representation of a coronal section of rat’s brain highlighting the dorso ventral range of recording and electrodes placement in the gustatory cortex for the right (light blue) and left (light orange) hemisphere. (B) Sketch of the experimental design for each trial. Red bars highlight stimulus delivery. See experimental procedures for further details. Each tastants is delivered through the IOCs in aliquots of 40 µl. Each cross modal stimulus is presented for 2 s. Between the cross modal stimuli offset and sucrose onset there is a constant delay of 500 ms.
DOI: http://dx.doi.org/10.7554/eLife.16420.011
Figure 3—figure supplement 2. The neural bias for somatosensory and olfactory stimuli does not depend on the number of sessions at final performance level.

Figure 3—figure supplement 2.

(A) Percentage of GC neurons modulated by cross-modal stimuli after matching the number of recording session at final performance level. (B) Distribution of the number of cross-modal neurons across recording days following reaching final performance level. Each full circle represents the number of responsive neurons recorded for each experimental day. Different colors represent responses to different cross-modal stimuli (see legend). Straight lines represent the best linear fit through each point.
DOI: http://dx.doi.org/10.7554/eLife.16420.012