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Validation of CpTRPA5-splice forms in male and female body parts by RT-PCR. For comparison, the full-length CpTRPA5 gene was also included. The housekeeping gene rpl8 was used as a positive control in all body parts. Ant: antennae; Tho: thorax; Abd: abdomen; Leg: legs; Win: wings; ntc: no-template control; M: male; F: female. PCR volume loaded: 10 μl.
