Table 1.
Sequences and estimated Tm for primers
| RACE primer (5′ or 3′) | Sequence | Tm (°C) |
|---|---|---|
| 5′_CpTRPA5_1 | AGCGGAACTGGATCATGAAG | 64.3 |
| 5′_CpTRPA5_2 | GAGATGGTGATGGCTGCAGGAAGGAGGG | 65.0 |
| 3′_CpTRPA5-1 | CAGGAAAACCAAGATGGAGGCACG | 66.9 |
| 3′_CpTRPA5-2 | GAGACGCCATTTTAGACAAAGCTCAAGCTC | 63.5 |
|
CDS extension (Fw or Rv) | ||
| Fw_CpTRPA5 | attB1-ATGGCAGCTTTATCAGGCGGCG | 65.8 |
| Rv_CpTRPA5 | attB2-TTATTTACTTAACTTACTTTCTAATCTTAACAA | 61.4 |
|
Sequencing primers (Fw or Rv) | ||
| M13 Fw | GTAAAACGACGGCCAGT | 52.4 |
| M13 Rv | CAGGAAACAGCTATGACC | 53.8 |
| Seq1-Fw | ATGATGGAGAGACTCCAATCCATTC | 64.1 |
| Seq2-Fw | ATGGGCTGGTTCCCTTTACATACAG | 65.8 |
| Seq3-Fw | TGCTGGCATGGTTAGAGATG | 58.4 |