Table 2.
List of forward primers designed to verify the existence of candidate CpTRPA5 splice forms
| Splice form | Forward primer | Tm (°C) |
|---|---|---|
| CpTRPA5_M4* | TAGACTTGCAAAACAATTTG | 50.2 |
| CpTRPA5_M17* | AAGTTTGGCTCCATCGGCC | 59.5 |
| CpTRPA5_M41 | ACTGACGGCCCTAAGAAATTC | 59.5 |
| CpTRPA5_M43* | CTCGTATTGATTCAGGAAAAC | 54.4 |
| CpTRPA5_M415 | TGGAAGAAGTTTTAGACTTGC | 55.4 |
| CpTRPA5_M417 | TTTATCTACGTTTGTGGCGTT | 55.4 |
| CpTRPA5_M418* | TAGTTTTAGGTACCTATAAGC | 53.0 |
| CpTRPA5_F1111 | TTAGTTGAGAGTTTCCTAACT | 53.4 |
| CpTRPA5_F1115 | TTGTTGCTAAAAGATGGCGCC | 59.5 |
| CpTRPA5_F1117* | TTTTACACTATTATAGCCATT | 49.0 |
| CpTRPA5_F1124 | TGAATACTTGGAAGAAGTTTA | 51.7 |
*Splice forms verified by RT-PCR.