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. 2016 Aug 25;16(1):83. doi: 10.1093/jisesa/iew072

Table 3.

List of forward and reverse primers designed to validate the expression of candidate Cp-TRPs

Gene Forward primer Reverse primer Tm (°C)
CpTRPA5 ATGGGCTGGTTCCCTTTACATACAG TTATTTACTTAACTTACTTTCTAATCTTAACAA 61.4
CpPyx TACCCAGCGTTCCAACTACC CATGAGAGCAGCGAACTGAA 63.2
CpWtrw TAGCCGGTTACTCCACCATC AAAACAGGGGAGGGTCATTC 63.2
CpTRP ATTCCCTCAGGCACTCACAA CATGAAAGCTGGAAGGCTGT 64.3
CpTRPC GGGAGACCAAGTCAACGGTATGC GATGCGTTCAGTGTACGTGTGC 65.4
CpOrco CCGGAGCCCACTGATATAGA CCTCAGAACCGTCGTACCAT 64.3