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. Author manuscript; available in PMC: 2016 Sep 16.
Published in final edited form as: Science. 2015 May 21;348(6240):1268–1270. doi: 10.1126/science.aaa2850

Fig. 2. Knockout with CRISPR-Cas9 demonstrates that Nix is required for male development.

Fig. 2

(A) Phenotypes of injected individuals. (B) The female isoform of dsx (dsxF) is present in nix− A. aegypti detected by ddPCR. Rel. Exp., relative expression. Error bars, mean ± SEM. (C) The female isoform of fru (fruF) is present in the nix− A. aegypti detected by ddPCR. Error bars, mean ± SEM. (D) Feminization of the antennae in a nix− male individual. (E) Feminization in the genitals of a nix− male individual. (F and G) The log2 reads per kilobase per million mapped reads expression level heat map of the top 100 male-biased (F) and female-biased (G) genes in wild-type males, nix− males, and wild-type females. Two heat maps from nix− males are shown here. All other heat maps are shown in fig. S10.