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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Crit Care Med. 2016 Oct;44(10):e973–e979. doi: 10.1097/CCM.0000000000001817

Figure 2. Selective depletion of Kupffer cells and in vivo Kupffer cell p38 inhibition.

Figure 2

(A) Liver sections immunostained for CD68 from mice receiving empty liposomes. (B) Liver sections immunostained for CD68 from mice receiving clodronate liposomes. (C) Peripheral blood monocytes. (D) Lung sections stained for alveolar macrophage marker SiglecF from mice receiving empty liposomes. (E) Lung sections stained for alveolar macrophage marker SiglecF from mice receiving clodronate liposomes. (F) p38 activation in isolated Kupffer cells from animals of the Burn + Ethanol Subgroup with and without LPS stimulation. Within the subgroup, animals given in vivo SB203580 (p38i) or vehicle control are shown. (G) IL-6 production in isolated Kupffer cells from animals of the Burn + Ethanol Subgroup with and without LPS stimulation. *p<0.05 by Student’s T test. N=3–6 animals per group.