Figure 3. Free serum hemoglobin (Hgb) on day 4.
As an index of hemolysis, serum hemoglobin was studied in the groups. Treatment with PBS or 1C10 followed by Stx-2 injection resulted in a significant increase in free Hgb compared to mice injected with only PBS + PBS or toxoid or treated with 3F8 then injected with Stx-2. Thus, MBL2 inhibition significantly prevented hemolysis. Bars and brackets represent mean ± SEM and numbers in the bars represent number of mice studied. *p<0.05 compared to PBS, toxoid or 3F8 + Stx-2.