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. Author manuscript; available in PMC: 2017 Sep 16.
Published in final edited form as: ACS Chem Biol. 2016 Jul 19;11(9):2506–2518. doi: 10.1021/acschembio.6b00383

Figure 6. The high-conductance, channel-forming ability of Wzm is impacted by Wzt.

Figure 6

(A) A representative electrical current recording showing two single-channel insertions of Wzm into a planar the lipid bilayer in the presence of Wzt. They are marked by the arrows 1 and 2; (B) A continuation of the same recording presented in A, showing a single closure of Wzm; (C) A histogram of the probability (P(G)) of the occurrence of a given single-channel conductance insertion (G(nS)) of Wzm in the presence of Wzt. A total of 25 channel insertions were used to construct this histogram. 50 ng Wzm and 500 ng Wzt proteins were incubated for 5 minutes. Thereafter, this protein mixture was added to the cis side. In this figure, the electrical traces were low-pass, Bessel filtered at 0.5 kHz. Other experimental conditions were the same as those in Fig. 2. The dashed lines represent the zero-current level.