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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Glia. 2016 Jul 29;64(11):1987–2004. doi: 10.1002/glia.23037

Fig. 4. EGF administration promotes OPC proliferation and maturation.

Fig. 4

A) Representative immunofluorescence of cryosections from a 3 d old pup double-labeled with PDGFRα and BrdU specific antibodies. Graphs show mean ± SEM (n = 5 each). Cycling PDGFRα+ cells (arrowhead) were reduced in pups with IVH, and rhEGF treatment increased their density. B) Representative immunofluorescence of cryosections from a 3 d old pup double-labeled with Olig2 and BrdU specific antibodies. Bar-charts are mean±SEM (n= 5 each). Note total and cycling Olig2+ cells show a trend toward reduction in pups with IVH (compared to no IVH controls) and an increase in rhEGF treated pups relative to vehicle controls. Arrowhead indicated Olig2+BrdU+ cells. C) Cryosections were double labeled with Nkx2.2 and Olig2 antibodies. Graphs show mean±SEM (n=5 each). Note reduced number of cells co-labeled with Nkx2.2+Olig2+ (arrowhead) in pups with IVH compared to vehicle controls, and rhEGF treatment increased the number in this subset of cells. *P<0.05, ***P<0.001 pups with vs. without IVH. #P<0.05, ##P<0.01, ###P<0.001 vehicle vs. rhEGF treated pups with IVH. Scale bar, 20μm.