Skip to main content
. Author manuscript; available in PMC: 2017 Sep 16.
Published in final edited form as: ACS Chem Biol. 2016 Jul 14;11(9):2475–2483. doi: 10.1021/acschembio.6b00415

Figure 2.

Figure 2

One-bead-one-compound library design and screening. (A) UNC3866-derivative library was designed to test structural hypotheses at all six positions of the parent compound. A constant linker containing methionine was included to allow for hit cleavage via cyanogen bromide treatment. (B) Library screening cascade used to selectively target CDYL2 which includes negative selections of homologous chromodomains (MPP8, CBX8, CBX7, and CBX5) on CDYL2 hit beads. Soluble UNC3866 was used to select for only higher potency ligands.