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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: Cancer Res. 2016 Jul 28;76(18):5442–5454. doi: 10.1158/0008-5472.CAN-15-3317

Figure 5.

Figure 5

Birinapant plus death ligands induced cell death in UM-SCC lines via caspase and/or necroptosis dependent mechanisms. A, Cell lines were treated with combinations of 1 μM birinapant, 20 ng/mL TNFα, 50 ng/mL TRAIL, 20 μM ZVAD (pan-caspase inhibitor), 20 μM ZIETD (caspase-8 inhibitor), or 20 μM necrostatin-1 (RIPK1 inhibitor) and assessed for effect on cell density or caspase-3 and -8 activation. Standard error bars for 3 replicates, Student t-test, *p<0.05. B, In independent experiments, UM-SCC-1, -46, and -11B cell lines were treated with 0.01% DMSO diluent control, or 1 μM birinapant and 20 ng/mL TNFα (B+T), and whole cell lysates procured 12, 24, and 48 hours after treatment were subjected to SDS-PAGE and Western blots. Expression for each protein was quantified and normalized to both diluent control (=1) and β-tubulin loading control for the respective lane for the blots shown for each cell line.