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. Author manuscript; available in PMC: 2017 Sep 16.

Published in final edited form as: ACS Chem Biol. 2016 Aug 5;11(9):2642–2654. doi: 10.1021/acschembio.6b00479

Table 3.

Steady-state Kinetic Parameters for MycCI-RhFRED^a

Substrate	Product formation			NADPH consumption

	K_cat (min⁻¹)	K_m (μM)	K_cat/K_m (μM⁻¹min⁻¹)	K_cat (min⁻¹)	K_m (μM)	K_cat/K_m (μM⁻¹min⁻¹)
8	0.24 ± 0.02	264 ± 50	0.0009 ± 0.0002	5.4 ± 0.2	99 ± 8	0.055 ± 0.005
9	1.15 ± 0.02	2.1 ± 0.4	0.6 ± 0.1	8.0 ± 0.3	1.5 ± 0.4	5 ± 1
10	0.355 ± 0.003	28 ± 1	0.0127 ± 0.0006	6.38 ± 0.08	14.9 ± 0.9	0.43 ± 0.03
11	0.88 ± 0.04	0.9 ± 0.5	1.0 ± 0.5	9.1 ± 0.3	1.5 ± 0.3	6 ± 1
12	0.72 ± 0.03	3.9 ± 0.8	0.19 ± 0.04	8.2 ± 0.1	1.0 ± 0.1	8 ± 1

^a

Reported errors are those obtained from fitting data averaged from experiments performed at least in triplicate.