. Author manuscript; available in PMC: 2017 Sep 16.

Published in final edited form as: ACS Chem Biol. 2016 Aug 5;11(9):2642–2654. doi: 10.1021/acschembio.6b00479

Table 4.

Turnover Frequencies (TOFs) for Selected Enzymatic Reactions^a

Catalytic system	Substrate
Catalytic system	8	9	10	11	12
MycCI/MycCII^b	0.97 ± 0.03	1.4 ± 0.1	0.96 ± 0.01	1.23 ± 0.05	1.11 ± 0.08
MycCI-RhFRED	0.161 ± 0.008	1.19 ± 0.06	0.34 ± 0.01	0.81 ± 0.01	0.61 ± 0.02
TylHI/TylHII^b	N.D.^c	N.D.	N.D.	N.D.	0.14 ± 0.03
TylHI/MycCII^b	N.D.	N.D.	N.D.	N.D.	1.63 ± 0.03
TylHI-RhFRED	N.D.	N.D.	N.D.	N.D.	0.76 ± 0.02
MycCI_S172A/MycCII^b	1.15 ± 0.05	1.09 ± 0.05	0.96 ± 0.02	0.90 ± 0.03	0.86 ± 0.06

TOF = mol product/mol P450 per min. Product formation was monitored by HPLC over the first 30 min of the reaction (initial rates). Reported errors are standard deviations calculated from experiments performed in triplicate.

Includes MBP-FdR.

N.D. = value not determined.