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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Virology. 2016 Aug 4;497:233–243. doi: 10.1016/j.virol.2016.07.025

Figure 1. US28 RNA and protein expression in HCMV infected THP-1 cells.

Figure 1

(A) THP-1 cells were infected with TB40E-US28FL virus or TB40E-ΔUS28 viruses at MOI of 10. Cells were harvested at days 1, 2, and 4 post-infection. RNA samples were converted into cDNA by reverse transcription. PCR was performed to analyze the expression of HCMV US28, immediate-early genes, and cellular actin (internal control). (B) THP-1 cells were infected with TB40E-US28FL virus or UV-inactivated virus at MOI of 10. Cells were harvested at days 1, 2, and 4 post-infection. Whole cell lysates were analyzed by western blot to detect HCMV immediate early gene (IE) protein, UL44, and actin (internal control). Immunoprecipitation and western blot with anti-flag antibody were performed to analyze US28 protein expression. d.p.i: days post infection.