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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Virology. 2016 Aug 4;497:233–243. doi: 10.1016/j.virol.2016.07.025

Figure 4. US28 induced PLC-β signaling in stable THP-1 lines is blocked by Gαq and PLC-β inhibitors but not Gαi inhibitors.

Figure 4

Transduced THP-1 cells were labeled with 3H-myoinositol 1 day before induction, and treated with 1 μg/ml of doxycycline or vehicle (H2O) for 24 hours. THP-1 cells were pretreated with inhibitors of Gαq (YM-254890, 100 nM), PLC-β (U73122, 2 μM), or Gαi (pertussis toxin, 200nM) for 30 minutes before IP3 assay. Percentage of IP3 conversion was calculated using the reading from column elution divided by the measure of total labelling. The graph represented three independent experiments. Statistical significance was determined by performing unpaired two-tailed Student’s t tests with GraphPad Prism® software. YM: YM-254890, PTX: pertussis toxin, ***: p=0.001,**:p<0.01, *: p<0.05, n.s.: not statistically significant.