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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Virology. 2016 Aug 4;497:233–243. doi: 10.1016/j.virol.2016.07.025

Figure 5. US28 expression in monocytes drives increased monocyte to endothelial cell adhesion.

Figure 5

Transduced THP-1 monocyte cells were treated with doxycycline (1 μg/ml) for 24 hours to induce US28 expression. Cells were then co-cultured with an endothelial cell monolayer (HECV) for indicated times. Unattached cells were removed by vigorous washing with media alone. The number of attached cells were counted by flow cytometry and calculated as a percentage of input cells used in the assay. The data represent 3 independent experiments. Statistical significance was determined by performing unpaired two-tailed Student’s t tests with GraphPad Prism® software. ** : p<0.01, *** : p<0.001