Skip to main content
. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Virology. 2016 Aug 4;497:233–243. doi: 10.1016/j.virol.2016.07.025

Figure 6. US28 induced monocyte-endothelial cell adhesion is dependent on constitutive G-protein based signaling but not the extracellular ligand binding domain.

Figure 6

Transduced THP-1 cells were treated with 1 μg/ml of doxycycline or vehicle (H2O) for 24 hours to induce US28 expression. 5×105 THP-1 cells were then co-cultured with HECV cells for one hour, and unattached cells were washed away with media. Numbers of input cells and cells attaching to HECV cells were determined by flow cytometry. The ratios between cells attaching to HECV cells and input cells were converted into a percentage. The graph represented eight independent experiments. Statistical significance was determined by performing unpaired two-tailed Student’s t tests with GraphPad Prism® software. ***: p<0.0001, *: p<0.05, n.s.: not statistically significant.