Figure 6. Altered TGF-β signaling in p53-KO T cells.

A) Activated h3T or h3T-p53 KO splenic CD8⁺ T cells obtained after FACS sorting were used to prepare RNA and determine the expression of TGF-β receptors (TGF-βRI and TGF-βRII). B) h3T or h3T-p53 KO splenic T cells were cultured for three days under iTreg polarizing conditions, and FoxP3 expression analysis was done using intracellular staining (left panel), or real-time PCR (right panel). C) RNA from A) was also used to run the TGF-β signaling real-time PCR based 84 gene array. The data obtained is presented in fold change with genes grouped for pathways indicated on left. The data is representative from one of two experiments and genes with similar results in both array experiments are presented.