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ELISA for VACV and MPXV dsRNA levels. HeLa cells were infected with VACV and MPXV at an MOI 5. At 1, 2, 4, 6, 8, 10, and 12 hpi cells were harvested, total RNA was extracted, and 500 ng of total RNA was added to ELISA plate. Signal was quantified by Luminescent plate reader. Data presented are means with standard error of multiple experiments.
