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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Virology. 2016 Aug 11;497:328–336. doi: 10.1016/j.virol.2016.07.029

Figure 4.

Figure 4

Capture of HIV-2iGFP Virions by Mature DCs is Attenuated. (A) Mature DCs were challenged with increasing amounts (ng of GFP) of HIV-1iGFP (blue) or HIV-2iGFP (red) virions at 37°C for 2 hours, washed, fixed and the percentage of GFP+ cells quantified using flow cytometry. The data shown is the mean ± SEM from 3 independent donors. Nonlinear regression was used to estimate a fitted curve in GraphPad Prism 5 (B) Mature DCs were challenged with 10 ng of GFP containing HIV-1iGFP or HIV-2iGFP virus particles in the absence (NT) or presence of anti-CD169 monoclonal antibody (10 μg/ml) at 37°C for 2 hours, washed, fixed and the percentage of GFP+ cells quantified using flow cytometry. (C) THP1/CD169 cells were challenged with 8.3 ng of GFP containing HIV-1iGFP or HIV-2iGFP virions at 37°C for 2 hours, washed, fixed and the percentage of GFP+ cells quantified using flow cytometry. The data shown in panels B and C is the mean percentage of GFP positive cells ± SEM of 5 or 7 independent experiments, respectively; *p=0.04. ***p=0.0008.