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. Author manuscript; available in PMC: 2017 Sep 16.
Published in final edited form as: Circ Res. 2016 Aug 11;119(7):827–838. doi: 10.1161/CIRCRESAHA.116.308856

Figure 3. ABCA1 flops PIP2.

Figure 3

A. Cell surface PIP2 assessed by flow cytometry in RAW264.7 cells ± ABCA1 induction that were pretreated ± PI-PLC (RFI, relative fluorescence intensity; mean ± SD; different letters show p<0.001 by ANOVA Bonferroni posttest, n=3). B. Western blot of RAW264.7 cell extracts showing expression of ABCA1was not altered by PI-PLC treatment. C. ABCA1 expression redistributed the PIP2 reporter, 2XPH-PLCδ-eGFP, away from the plasma membrane in live stably transfected RAW264.7 cells. D. Percentage of PIP2 reporter association with plasma membrane ± ABCA1 induction (n=10 and 20 for control and ABCA1 expressing cells, respectively).