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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Virology. 2016 Aug 16;497:346–356. doi: 10.1016/j.virol.2016.07.028

Figure 5. H2AX is not required for recruitment of DDR proteins, virus production, or viral DNA accumulation during MuPyV infection.

Figure 5

H2AX-null (H2AX-/-) fibroblasts were infected with NG59RA, and at 28 HPI cells were fixed and immunostained. Single z-plane confocal images of cells stained for A) Viral DNA and Tag, B) Viral DNA and Mre11, or C) Tag and pCHK1. D) H2AX WT (H2AX +/+) and null (H2AX-/-) cells were infected with NG59RA. Virus supernatants were harvested at 48 HPI and viral titers were quantified by immunoplaque assay, normalized to the value of the control or E) viral DNA was harvested at 32 HPI and viral DNA accumulation was quantified by qPCR, normalized to the value of the control.