Fig. 6.

In vitro neuroprotection by LIF is dependent upon Akt activity. a LIF treatment significantly lowered neuronal death during OGD compared to PBS (*P < 0.001). b Akt IV reverses the neuroprotective effect of LIF treatment alone (#P < 0.01). n = 12 per treatment group. Representative images show B p-Akt and C SOD3 immunoreactivity in neurons under normoxia. DMSO + PBS treatment before 24 h OGD increased D p-Akt and E SOD3 levels compared to 24 h normoxia. F Phospho-Akt (Ser473) and G SOD3 levels were highest in cells treated with DMSO + LIF before OGD. Addition of Akt IV + PBS prior to OGD caused H a minimal decrease in phospho-Akt (Ser473) and I no change to SOD3 levels compared to the DMSO + PBS group. Co-incubation with Akt Inhibitor IV + LIF prior to OGD prevented the LIF-induced increase in J phospho-Akt (Ser473) and K SOD3 expression. Arrows identify representative cells. P-Akt phospho-Akt (Ser473), Akt IV Akt Inhibitor IV. Scale bar = 25 μm