Table 1.
Oligonucleotide primer sequences used for qPCR in the present study
| Gene name | Accession no. | Primer | Product Size (bp) |
|---|---|---|---|
| GDF9 | NM_005260.4 | ||
| Sense | CCAGGTAACAGGAATCCTTC | 162 | |
| Antisense | GGCTCCTTTATCATTAGATTG | ||
| BM15 | NM_005448.2 | 129 | |
| Sense | CCTCACAGAGGTATCTGGC | ||
| Antisense | GGAGAGATTGAAGCGAGTTAG | ||
| ATPase 6 | YP_003024031.1 | 123 | |
| Sense | CTGTTCGCTTCATTCATTG | ||
| Antisense | GGTGGTGATTAGTCGGTTG | ||
| NAIP | NM_004536.2 | 184 | |
| Sense | GGAGTATTTGGATGACAGAAAC | ||
| Antisense | TAGATTACCACTGGAGTCTTCC | ||
| TP53 | NM_000546.5 | 184 | |
| Sense | GGAGTATTTGGATGACAGAAAC | ||
| Antisense | TAGATTACCACTGGAGTCTTCC | ||
| BUB1 | NM_001278616.1 | 100 | |
| Sense | AAGGTCCGAGGTTAATCC | ||
| Antisense | CACTGGTGTCTGCTGATAGG | ||
| MADL2 | NM_002358.3 | 169 | |
| Sense | CTTCTCATTCGGCATCAAC | ||
| Antisense | ACACTTGTATAACCAATCTTTCAG | ||
| CDC20 | NM_001255.2 | 202 | |
| Sense | GATGTAGAGGAAGCCAAGATC | ||
| Antisense | CCACAAGGTTCAGGTAATAGTC | ||
| ATR | NM_001184.3 | 150 | |
| Sense | GATGCCACTGCTTGTTATG | ||
| Antisense | CCACTCGGACCTGTTAGC | ||
| ATM | NM_000051.3 | 107 | |
| Sense | GCATTACGGGTGTTGAAG | ||
| Antisense | ATATAGAAGGACCTCTACAATG | ||
| BRCA1 | NM_007294.3 | 141 | |
| Sense | CACTCAGCAGAGGGATACC | ||
| Antisense | TCAAGGGCAGAAGAGTCAC | ||
| AURKC | NM_001015878.1 | 176 | |
| Sense | CGCACAGCCACGATAATAG | ||
| Antisense | CACATTGTCTTCCTCCTCAG | ||
| CDC25A | NM_001789.2 | 96 | |
| Sense | CTTTATGAAATGCCAGTCTTAC | ||
| Antisense | CTCTTGGTGCGGAACTTC | ||
| CDC25B | NM_001287516.1 | 120 | |
| Sense | TGACTTAAAGGATGATGATGC | ||
| Antisense | CGCTGGCACTTGCTGTAC | ||
| β.actin | NM_001101.3 | 90 | |
| Sense | CAAGATCATTGCTCCTCCTG | ||
| Antisense | ATCCACATCTGCTGGAAGG |