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. 2016 Sep 14;20(3):392–405. doi: 10.1016/j.chom.2016.08.005

Figure 7.

Figure 7

Multiple Viral Determinants and ISGs Underlie Inhibition of HIV-1 Replication by Endogenous TRIM56

(A and B) Spreading replication of HIV-1 and HIV-1/TRIM56R in GHOSTX4-vector and GHOSTX4-TRIM56#2 cells (A), or chimeric viruses constructed using HIV-1 and HIV-1/TRIM56R (B).

(C) WB analysis of TRIM56 expression in clones of MT4-LTR-GFP cells transduced with lentiviral vectors expressing Cas9 only or Cas9 plus one of two TRIM56-targeted guide RNAs (CR1 or CR3).

(D and E) HIV-1 replication in the absence (D) or presence (E) of 25U/ml IFNα in clones of control (n = 4) or TRIM56 knockout (n = 7) MT4-LTR-GFP cells described in (C).

(F and G) Microarray analysis of the expression levels (in a.u.) for the ∼120 genes most highly induced by 25U/ml IFNα in clones of control (n = 4) or TRIM56 knockout (n = 7) MT4-LTR-GFP cells described in (C) in the presence (G) or absence (F) of IFN.