Fig. 4.

Transcriptional regulatory activity of MIR ECRs: a) Regulatory function of ECR sequences was assessed by dual luciferase assay in the pGL3P reporter vector in SH-SY5Y neuroblastoma cells under basal conditions. All ECRs tested displayed regulatory properties in vitro, with 5 showing positive regulatory function and the remaining two displaying negative regulatory effects. N = 4; *P < 0.1, **P < 0.01, ***P < 0.001. b) Schematic of the MIR 1 ECR and flanking region, showing the human EST, AW901379. AceView (http://www.ncbi.nlm.nih.gov/ieb/research/acembly/) lists this EST as being identified in nervous tissue, and data from Fig. 3a showed histone modifications around the MIR 1 ECR indicative of active transcription from this locus in the human brain