Table 1. Variability of 10 polymorphic microsatellite loci in santer Cheimerius nufar from South Africa (n=131) grouped into two multiplex reactions (I and II).
| Locus name | GenBank accession number | Dye label | Primer sequences (5′–3′) | Repeat motif | Ta | Size range (bp) | NA | He | Ho |
|---|---|---|---|---|---|---|---|---|---|
| SA1 (I) | 6FAM | F:CAGCGATGCACAGTAAAGTACC R:AGCATACAGAGGCCTTCAGC | (TG)33 | 58 | 253–293 | 19 | 0.93 | 0.94 | |
| SA2 (II) | NED | F:GAGCCAGACTCCAGACATCC R:CCGGACAGGAGTATTGAAGC | (GTCT)11 | 58 | 190–234 | 8 | 0.75 | 0.73 | |
| SA3 (I) | PET | F:CCAGAGTCTGTGCTGTGAGTGC R:TCCTTGTGGTCCACTTTACG | (CA)17 | 58 | 386–420 | 13 | 0.89 | 0.77 | |
| SA6 (I) | 6FAM | F:AGCTGCTGCTCATCTCACG R:GCAGTGTTAACATCTTCGAATGC | (TG)12 | 58 | 187–209 | 9 | 0.75 | 0.79 | |
| SA10 (I) | 6FAM | F:GAAGCCAAACGAGGACAGC R:GTGAGGAGCATGCTAATACCG | (GT)15GA(GT)26 GAGTGAGTGA(GT)18 | 58 | 428–532 | 42 | 0.96 | 0.92 | |
| SA25 (I) | VIC | F:GGAGGAAATGAACCGATGG R:GCAGCTGGTCAATAGTGTGG | (TG)7CA(TG)6 | 58 | 152–220 | 19 | 0.61 | 0.70 | |
| SL25 (II) | FJ526983 | NED | F:GGTACTGTTTGGCCCTTGC R:GCCTGGTAATATGCCTGAGC | (GA)11TG(GA)3TA(GA)4GTCA (GA)2AA(GA)9CA(GA)2AA(GA)12 | 58 | 207–271 | 7 | 0.61 | 0.66 |
| SL26 (I) | FJ526984 | PET | F:TGAAGGTGCTGATGACTTTCC R:CAGTCCTGCCTCTGACTGG | (TC)2TT(TC)9GC(TC)4TGCCTT (TC)5GC(TC)7 | 58 | 239–247 | 5 | 0.38 | 0.32 |
| SL27 (I) | FJ526985 | VIC | F:CAGCCTCAGCTCATTTCTCC R:CCTGCCCTCCTGTAGATGC | (TG)55 | 58 | 183–201 | 6 | 0.35 | 0.32 |
| SL34 (I) | FJ526988 | VIC | F:GCGTGCACACTCTTACAGTACC R:TCGGATGTGCATCTCATAGG | (CA)17 | 58 | 321–361 | 19 | 0.84 | 0.85 |
Abbreviations: He, expected heterozygosity; Ho, observed heterozygosity; NA, number of alleles observed; Ta, annealing temperature (°C).
For slinger Chrysoblephus puniceus, see Chopelet et al. (2009a).