Figure 4.

Detection of avian influenza virus nucleoprotein in infected chickens using immunohistochemistry Detection of influenza- nucleoprotein by immunohistochemistry (ABC method, intranuclear and intracytoplasmis bright red antigen signal by 3-amino-9-ethyl-carbazol chromogen and haematoxylin (blue) counterstain): heart (a, d, g), lung (b, e, h) and brain (c, f, i) samples obtained from birds infected with Hp-NS224 (a-c), HP- NS230 (d-f) and HP-NSLp (g-i) at 4 d post infection; for detailed data on the full tropism to different organs examined in this study readers are refered to Table S2. Bar = 50 µm.