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. 2016 Aug 5;15(18):2420–2430. doi: 10.1080/15384101.2016.1181242

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© 2016 Taylor & Francis

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Figure 1. — Anti-21A RNAs transfection leads to a transient cell proliferation boost. (A) Quantification of red-labeled Anti-21A RNAs signal in S1 neuroblastoma cells at different time points (seven microscope fields were averaged for each time point). Microscope fields here reported represent S1 cells 24 hours after Anti-21A RNAs transfection. Scale bar: 200 μm. (B) Real time RT-PCR analysis of interferon response biomarkers in S1 cells transfected with Anti-21A RNAs or with water as control; gene expression levels are presented as normalized to GAPDH. Bulk Natural Killer population (NK) was used as positive control. (C) Population doubling of S1 cells transfected with Anti-21A RNAs or water as control, calculated in cell counting experiment. The inset represents the Population Doubling of S1cells replated 20 d after transfection with Anti-21A RNAs or water as control. (***p < 0.001).