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. 2016 Aug 5;15(18):2420–2430. doi: 10.1080/15384101.2016.1181242

Figure 2.

Figure 2.

Anti-21A RNAs-dependent proliferation boost does not lead to the acquisition of tumorigenic potential. (A) Real time RT-PCR analysis of oncogenes and oncosuppressors in S1 cells transfected with Anti-21A RNAs; results (gray columns) are normalized to the water-transfected samples (black columns). (B) Colony-forming potential in Methocult of S1 cells transfected with Anti-21A RNAs and/or water (control). Scale bar: 1000 μm. (C) Real-time monitoring migration and invasion of S1 cells transfected with Anti-21A RNAs using the xCELLigence Migration and invasion assay. Blue, Huvec FBS- cells; green, S1 FBS-, Azure, S1 Anti-21A RNAs-transfected FBS-; magenta, HUVEC FBS+; violet, S1 FBS+; orange, S1 Anti-21A RNAs-transfected FBS+. Cells were seeded and their migration was monitored for 24 hours. Cells maintained in serum-free media served as a control. (D) Analysis of secreted cytokines in S1 cells transfected with Anti-21A RNAs (empty bars) or water control (full bars) by MILLIPLEX technology; the amount of cytokines secreted after a 2° treatment of Anti-21A are reported (striped bars). (E) Real time RT-PCR expression analysis of stemness markers; results are reported as Anti-21A/water control ratio. (*p < 0.05; **p < 0.01).