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. 2016 Aug 5;15(18):2420–2430. doi: 10.1080/15384101.2016.1181242

Figure 3.

Figure 3.

Anti-21A RNAs-transfected MSCs implanted in vivo drive the differentiation of an increased portion of compact fibrous tissue. A) [3H]-thymidine incorporation assay of hMSCs transfected with different combination of α, β and γ Anti-21A RNAs and B) effects on cell proliferation of different concentrations of the equimolar mix (α +β +γ) of Anti-21A RNAs. C) Reconstituted Hematoxilin/Eosin (H/E)-stained skelite sections of different implant conditions and their 10x respective magnifications. D) 10x magnification of Hematoxilin/Eosin-stained sections analyzed under transmitted light (T/L) and under polarized light (P/L). Scale bar: 250 μm. E) Picrosirius red/Fast green-stained skelite sections analyzed under transmitted light (T/L) microscopy and polarized light (P/L) microscopy. Mature collagen type I is stained in red, collagen type I in the maturation process in yellow, and collagen type III in light green in polarized light. All samples were obtained 45 d post-implantation. Scale bar: 250 μm.