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. 2016 Jul 29;15(18):2454–2463. doi: 10.1080/15384101.2016.1201255

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Figure 1. — Aneuploidy is increased and has no effect on GVBD and PBE in aged oocytes. (A) Numbers analyzed are indicated above the bars. The data were analyzed using a chi-square test. The experiments were repeated more than 3 times. ** P < 0.01. (B) Kinetics of germinal vesicle breakdown (GVBD). GV-stage oocytes were isolated in M2 medium containing dbcAMP, which inhibits GVBD, and were released into inhibitor-free medium (at time = 0). The number of oocytes examined is indicated (n). (C) Kinetics of polar body extrusion (PBE). Oocytes that had undergone GVBD within 1.5 h after release into dbcAMP-free M16 medium were selected (at time = 0). The number of oocytes examined is indicated (n).