Figure 2.
Aging leads to an increase in oocyte pHi. (A) Full-grown and denuded GV oocytes were incubated with BCECF-AM to determine the pHi. Scale bar, 30 μm. (B) Full-grown GV oocytes were released from the ovaries of mice of different ages and were then removed to low-lactate KSOM to stabilize for 15 min before their pHi values were measured. (C) Oocytes from 1- and 12-month-old mice represent the young and aged groups, respectively. Full-grown GV oocytes were cultured in M16 medium for 2-2.5, 7.5-8 and 16-17 h to reach GVBD, MI and MII stages, respectively. In B and C, each point represents between 47 and 120 oocytes from 4 to 8 replicates. Data are presented as the means ± SEM.