Figure 3.
HCO3−/Cl− exchanger activity is attenuated with advancing age in full-grown GV oocytes. (A) Model for the increase in oocyte pHi using a Cl− removal medium. (B) pHi was monitored in denuded and full-grown GV oocytes from 1-, 3-, 6-, 9-, 12-, and 15-month-old mice; simultaneously, Cl− was removed from the bathing medium at 10 mins, indicated by the red arrow; Mins: minutes, M: month. Traces shown are the means of all experiments performed. (C) The rate of pHi increase upon C− removal in GV oocytes was quantified, providing an indication of HCO3−/Cl− exchanger activity. Each point represents between 47 and 120 oocytes from 4 to 8 replicates. (D) The relative AE2 mRNA expression levels in GV oocytes from 1- and 12-month-old mice. Gapdh served as the internal control gene. The data are expressed as the means ± SEM. ** P < 0.01. Each experiment employed 80 oocytes and was repeated 4 times.