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The expression level of SMC3 protein on chromosomes was obviously decreased in alkaline young oocytes. (A) The levels of chromosome-associated SMC3 protein were detected by immunofluorescence in normal, DIDS control and alkaline-treated oocytes at MI. Representative images show DNA (blue), CREST (red) and SMC3 (green). Scale bar, 5 μm. (B) SMC3 fluorescence intensity was quantified. Data are the means ± SEM, ** P < 0.01; ≥150 bivalents of each group were analyzed.
