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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: J Psychiatr Res. 2016 Jul 18;82:58–67. doi: 10.1016/j.jpsychires.2016.07.012

Figure 2. PDE4B, NCOA1 and NCOR2 are targets of miRNAs dysregulated in psychiatric illness.

Figure 2

HEK293 cell cultures were plated and cotransfected with a miRNA mimic (either miR-34a or miR-184) and a firefly luciferase vector (Yu, Chung, 2008) containing either a wild-type 3’ UTR (WT 3’ UTR) or a 3’ UTR mutagenized at a predicted miRNA binding site (34a-MUT or 184-MUT 3’ UTR) (n=6/group). A vector encoding renilla luciferase was used to normalize luciferase activity. Significant reductions in luciferase activity were observed for PDE4B when cotransfected with either miR-34a or miR-184 (Fig. 2a), NCOA1 when cotransfected with miR-34a (Fig. 2b) and NCOR2 when cotransfected with miR-184 (Fig. 2c). Mutagenesis of the predicted binding sites was sufficient to relieve these 3’ UTR constructs of miRNA-induced inhibition. Mutagenesis of sites in the NCOR2 3’ UTR was not performed since this UTR showed no significant repression by miR-34a. (*: P < 0.05, #: P ≤ 0.10 ; error bars represent SEM.)