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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Immunol. 2016 Aug 24;197(7):2909–2917. doi: 10.4049/jimmunol.1600651

FIGURE 3. IL-33 signaling is suppressed by lactic acid treatment.

FIGURE 3

BMMC were pretreated for 24 hours with media or lactic acid then activated with IL-33 at 200ng/mL for 5 minutes. Lysates were analyzed by western blotting to measure expression and phosphorylation of the indicated proteins. Representative blots are shown on the left, while bar charts show mean ± SEM of phospho-protein:total protein ratios calculated using the LI-Cor Odyssey software Image Studio 4.0. Results shown are representative of three experiments done in triplicate. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001; NS, not significant.