Skip to main content
. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Immunol. 2016 Aug 24;197(7):2909–2917. doi: 10.4049/jimmunol.1600651

FIGURE 4. Signal transduction inhibitors reproduce lactic acid-mediated suppression.

FIGURE 4

BMMC were pretreated with inhibitors (JNK: SP600125, 10 μM; TAK1: (5Z)-7-Oxozeaenol, 5 μM; NFκB: BAY 11-7085, 2 μM; ERK: FR180204, 25 μM) for 1 hour. Cells were then activated with 100ng/mL of IL-33 for 16 hours, supernatants were collected and analyzed via ELISA. Data are shown as mean ± SEM of three independent experiments conducted in triplicate. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001; NS, not significant.