Skip to main content
. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Immunol. 2016 Aug 24;197(7):2909–2917. doi: 10.4049/jimmunol.1600651

FIGURE 5. Lactic acid effects require HIF-1α-dependent miR-155-5p suppression.

FIGURE 5

A) BMMC were treated with lactic acid for 6 hours followed by collecting RNA to measure HIF-1α and miR-155 product. B) Cells were transfected with HIF-1α siRNA, incubated for 24 hours, treated with lactic acid for 6 hours, and then microRNA and mRNA were collected to measure miR-155-5p, as well as HIF-1α (to confirm knockdown). C) and D) BMMC were transfected with miR-155 mimics or a control/Mock transcript. After 48 hours of culture, cells were treated with LA for 24 hours, activated with IL-33 for 16 hours, and cytokines were measured by ELISA. Confirmation of transfection is shown in C) and examining the ability of LA to suppress IL-33-mediated cytokine production is shown in D). Results are expressed as mean ±SEM. Results in A) are 3 independent experiments conducted in triplicate and results in B), C), and D) are representative of 2 independent experiments done in triplicate. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001; NS, not significant.